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KMID : 0624620140470070411
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BMB Reports
2014 Volume.47 No. 7 p.411 ~ p.416
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Human selenium binding protein-1 (hSP56) is a negative regulator of HIF-1α and suppresses the malignant characteristics of prostate cancer cells
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Jeong Jee-Yeong
Zhou Jin-Rong
Gao Chong
Feldman Laurie
Sytkowski Arthur J.
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Abstract
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In the present study, we demonstrate that ectopic expression of 56-kDa human selenium binding protein-1 (hSP56) in PC-3 cells that do not normally express hSP56 results in a marked inhibition of cell growth in vitro and in vivo. Down-regulation of hSP56 in LNCaP cells that normally express hSP56 results in enhanced anchorage-independent growth. PC-3 cells expressing hSP56 exhibit a significant reduction of hypoxia inducible protein (HIF)-1α protein levels under hypoxic conditions without altering HIF-1α mRNA (HIF1A) levels. Taken together, our findings strongly suggest that hSP56 plays a critical role in prostate cells by mechanisms including negative regulation of HIF-1α, thus identifying hSP56 as a candidate anti-oncogene product.
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KEYWORD
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Hypoxia inducible factor, Prostate cancer, Protein-protein interaction, pVHL-interacting deubiquitinating enzyme, Selenium binding protein
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